GM501 SpermMobil

GM501 SpermMobil is a HEPES-buffered reagent containing low bicarbonate. It is used for in-vitro examination of sperms of necrozoospermic ejaculates as well as of immotile sperms isolated from testicular tissue (TESE).

Composition：

• NaCl, KCl, KH₂PO₄, MgSO₄^.7H₂O, NaHCO₃, CaCl₂^.2H₂O, D(+)-Glucose Anhydrous, Na-Lactate, Na-Pyruvate, EDTA, Alanyl-Glutamine, water, non-essential and essential Amino Acids, HEPES, Theophylline, Phenol Red.

Product specifications and quality control：

• All raw materials are of highest available purity (European Pharmacopoeia and/or USP standard) if applicable.
• A certificate of analysis is available for each batch upon request from our website with respective lot number.
• The MSDS for GM501 SpermMobil is available upon request and can be downloaded from our website.
• GM501 SpermMobil is manufactured and tested according to the following specifications:

Instructions for use:

• The intended users are IVF professionals (biologists, lab technicians, embryologists or medical doctors).
• Do not equilibrate GM501 SpermMobil in a CO₂-incubator, just warm it up to 37°C. GM501 SpermMobil is HEPES-buffered. Incubation in a CO₂-incubator will lower the pH.
• To facilitate sperm-activation add 3-4 μl GM501 Sperm­Mobil to the sperm-containing drop (approx. 30-40μl/ditution 1:10) in sperm preparation-medium inside the Petri-dishes.
• GM501 SpermMobil should be added to the opposing side from where the sperms are to be aspirated.
• Wait for 10 minutes. The activating effect initiates after a few minutes and lasts for up to one hour.

Precautions and warnings：

• Handle all specimens as if capable of transmitting HIV or hepatitis. Always wear protective clothing when handling specimens.
• Always work under strict hygienic conditions (ISO 5 environment, e.g. LAF-bench) to avoid possible contamination.
• Only for the intended use.

Pre-use checks：

• Do not use the product if bottle, seal of the container or package is opened or defective when the product is delivered.
• The concentration of the xanthine derivative Theophylline leads to the occasional formation of precipitate at 2-8°C storage temperature. It is dissolved completely when warmed at 37°C (within 24 h) and does not compromise function and safety of the product.
• Do not use the product if it becomes discoloured, cloudy or shows any evidence of microbial contamination.

Storage instructions and stability：

• Shelf life is 6 months from time of manufacture.
• Store between 2-8°C.
• Do not freeze before use.
• Keep away from (sun)light.
• The product can be used safely up to 7 days after opening, when sterile conditions are maintained and the products are stored at 2-8°C.
• Do not use after expiry date.